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KMID : 0363320130340020134
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Journal of Korean Oriental Internal Medicine
2013 Volume.34 No. 2 p.134 ~ p.146
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Effects of Auklandia Lappa on Dextran Sulfate Sodium-Induced Murine Colitis
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Kim So-Yeon
Park Jae-Woo
Ryu Bong-Ha
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Abstract
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Objectives : Auklandia Lappa (ALE) is one of the herbs used frequently to treat abdominal pain and diarrhea and reported
anti-inflammatory activity by suppressing proinflammatory cytokines. This study was designed to investigate whether ALE
could show protective activities on experimental colitis induced by dextran sulfate sodium (DSS) models.
Methods : Colitis was induced by DSS in Balb/c mice. ALE 10, 30, 100 and 300 mg/kg were orally administered twice a
day for 7 days in DSS model. Mice weight was measured daily. Scoring of clinical findings was measured every other day. Colon length, edema, fecal blood and histological damages were assessed at day 7 in DSS model. In histological analysis, we checked cryptal glands, surface epithelium, submucosa, transmural, stroma and scored degree of inflammatory cell damage by modified histological scoring. We also calculated cytokines concentrations including IFN-γ, TNF-α, IL-6, IL-1β, IL-17, IL-23, IL-10 and TGF-β1 by Biometric Multiplex Cytokine Profiling method.
Results : ALE showed the protective effects on DSS-induced experimental colitis. ALE inhibited shortening of colon length
and histological damages of colon does-dependently, but it did not inhibit weight loss. ALE also inhibited IFN-γ and IL-6
expression, and upregulated cytokines (IL-10, TGF-β1) related to regulatory T cell differentiation and proliferation.
Conclusions : The current results demonstrate the clinical utility of ALE in traditional medicine and indicate the possibility
of potent drug development of inflammatory bowel diseases from natural products. Further investigations for exact mechanisms will be needed.
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KEYWORD
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Auklandia Lappa, inflammatory bowel disease, dextran sulfate sodium, multiplex immunoassay
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